Nutrients, Vol. 17, Pages 5: The Phytochemical Composition and Antioxidant Activity of Matricaria recutita Blossoms and Zingiber officinale Rhizome Ethanol Extracts
Nutrients doi: 10.3390/nu17010005
Authors:
Anca Elena But
Raluca Maria Pop
Georg Friedrich Binsfeld
Floricuța Ranga
Meda Sandra Orăsan
Andra Diana Cecan
Iulia Ioana Morar
Elisabeta Ioana Chera
Teodora Irina Bonci
Lia Oxana Usatiuc
Mădălina Țicolea
Florinela Adriana Cătoi
Alina Elena Pârvu
Mircea Constantin Dinu Ghergie
Background: Inflammation-induced oxidative stress is a pathophysiological mechanism of inflammatory diseases. Treatments targeting oxidative stress can reduce inflammatory tissue damage. Objectives: This study aimed to conduct phytochemical analysis and evaluate the antioxidant effects of the hydroalcoholic extract of Matricaria recutita blossoms (M. recutita) and Zingiber officinale rhizomes (Z. officinale). Materials and Methods: The phytochemical analysis was carried out by measuring the total polyphenol content, total flavonoid content, and polyphenolic compounds’ HPLC-ESI MS. The antioxidant activity was evaluated in vitro through H2O2 DPPH, FRAP, and NO scavenging assays. An in vivo experiment was performed on rats with turpentine oil-induced acute inflammation. Treatments were administrated orally for 10 days, with three dilutions of each extract (100%, 50%, 25%), and compared to the CONTROL, inflammation, Diclofenac, and Trolox groups. In vivo, the antioxidant activity was evaluated by measuring the total antioxidant capacity (TAC), total oxidative status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein products (AOPP), and total thiols (SH). Results: The phytochemical analysis found a high content of phenolic compounds in both extracts, and the in vitro antioxidant activity was significant. In vivo, M. recutita and Z. officinale extracts proved to be effective in increasing TAC and lowering oxidative stress markers, respectively, the TOS, OSI, MDA, and NO levels. The effects were dose-dependent, with the lower concentrations being more efficient antioxidants. Matricaria recutita and Z. officinale extract effects were as good as those of trolox and diclofenac. Conclusions: Treatment with M. recutita and Z. officinale alleviated inflammation-induced oxidative stress. These findings suggest that M. recutita and Z. officinale extracts could be a promising adjuvant antioxidant therapy in inflammatory diseases.
Background: Inflammation-induced oxidative stress is a pathophysiological mechanism of inflammatory diseases. Treatments targeting oxidative stress can reduce inflammatory tissue damage. Objectives: This study aimed to conduct phytochemical analysis and evaluate the antioxidant effects of the hydroalcoholic extract of Matricaria recutita blossoms (M. recutita) and Zingiber officinale rhizomes (Z. officinale). Materials and Methods: The phytochemical analysis was carried out by measuring the total polyphenol content, total flavonoid content, and polyphenolic compounds’ HPLC-ESI MS. The antioxidant activity was evaluated in vitro through H2O2 DPPH, FRAP, and NO scavenging assays. An in vivo experiment was performed on rats with turpentine oil-induced acute inflammation. Treatments were administrated orally for 10 days, with three dilutions of each extract (100%, 50%, 25%), and compared to the CONTROL, inflammation, Diclofenac, and Trolox groups. In vivo, the antioxidant activity was evaluated by measuring the total antioxidant capacity (TAC), total oxidative status (TOS), oxidative stress index (OSI), malondialdehyde (MDA), nitric oxide (NO), advanced oxidation protein products (AOPP), and total thiols (SH). Results: The phytochemical analysis found a high content of phenolic compounds in both extracts, and the in vitro antioxidant activity was significant. In vivo, M. recutita and Z. officinale extracts proved to be effective in increasing TAC and lowering oxidative stress markers, respectively, the TOS, OSI, MDA, and NO levels. The effects were dose-dependent, with the lower concentrations being more efficient antioxidants. Matricaria recutita and Z. officinale extract effects were as good as those of trolox and diclofenac. Conclusions: Treatment with M. recutita and Z. officinale alleviated inflammation-induced oxidative stress. These findings suggest that M. recutita and Z. officinale extracts could be a promising adjuvant antioxidant therapy in inflammatory diseases. Read More